2013 ~ 2015 Research Institute of Pharmaceutical Sciences, Seoul National University (서울대학교 종합약학연구소 연구원)
2016 ~ 2022 University of Toronto / University Health Network, Canada (토론토대학병원 연구원)
2022 ~ 2024 차의과학대학교 약학대학 조교수
2024 ~ 2025 성균관대학교 약학대학 조교수
2025 ~ 현재 성균관대학교 약학대학 부교수
최근 연구업적
Lee, S. Y., Eun, H. J. & Lee, K. Y. Effector Binding Sequentially Alters KRAS Dimerization on the Membrane: New Insights Into RAS-Mediated RAF Activation. Advanced science, e2401530, doi:10.1002/advs.202401530 (2024).
Eun, H. J., Lee, S. Y. & Lee, K. Y. DNA binding reveals hidden interdomain allostery of a MazE antitoxin from Mycobacterium tuberculosis. Biochemical and biophysical research communications 710, 149898, doi:10.1016/j.bbrc.2024.149898 (2024).
Lee, K. Y. Membrane-Driven Dimerization of the Peripheral Membrane Protein KRAS: Implications for Downstream Signaling. International journal of molecular sciences 25, doi:10.3390/ijms25052530 (2024).
Lee, S. Y. & Lee, K. Y. Conditional Cooperativity in RAS Assembly Pathways on Nanodiscs and Altered GTPase Cycling. Angew Chem Int Ed Engl. 63, e202316942, doi:10.1002/anie.202316942 (2024).
Saitoh, T. et al. Biochemical and biophysical characterization of the RAS family small GTPase protein DiRAS3. Protein expression and purification 212, 106361, doi:10.1016/j.pep.2023.106361 (2023).
Lee, K.Y., Ikura, M., Marshall, C.B. The Self-Association of the KRAS4b Protein is Altered by Lipid-Bilayer Composition and Electrostatics. Angew Chem Int Ed Engl. 62(18):e202218698 (2023).
Lee, K. Y. & Lee, B. J. Dynamics-Based Regulatory Switches of Type II Antitoxins: Insights into New Antimicrobial Discovery. Antibiotics 12, doi:10.3390/antibiotics12040637 (2023).
Lee, K. Y. et al. Oncogenic KRAS G12D mutation promotes dimerization through a second, phosphatidylserine-dependent interface: a model for KRAS oligomerization. Chemical science 12, 12827-12837, doi:10.1039/d1sc03484g (2021).
Marshall, C. B. et al. NMR in integrated biophysical drug discovery for RAS: past, present, and future. Journal of biomolecular NMR 74, 531-554, doi:10.1007/s10858-020-00338-6 (2020).
Lee, K. Y. et al. Structural and functional study of SaAcP, an acylphosphatase from Staphylococcus aureus. Biochemical and biophysical research communications 532, 173-178, doi:10.1016/j.bbrc.2020.07.068 (2020).
Lee, K. Y. et al. Two Distinct Structures of Membrane-Associated Homodimers of GTP- and GDP-Bound KRAS4B Revealed by Paramagnetic Relaxation Enhancement. Angewandte Chemie 59, 11037-11045, doi:10.1002/anie.202001758 (2020).
Eun, H. J., Lee, K. Y., Kim, D. G., Im, D. & Lee, B. J. Crystal structure of the YoeB(Sa1)-YefM(Sa1) complex from Staphylococcus aureus. Biochemical and biophysical research communications 527, 264-269, doi:10.1016/j.bbrc.2020.04.089 (2020).
Fang, Z. et al. Multivalent assembly of KRAS with the RAS-binding and cysteine-rich domains of CRAF on the membrane. Proceedings of the National Academy of Sciences of the United States of America 117, 12101-12108, doi:10.1073/pnas.1914076117 (2020).
Grant, B. M. M. et al. Calmodulin disrupts plasma membrane localization of farnesylated KRAS4b by sequestering its lipid moiety. Science signaling 13, doi:10.1126/scisignal.aaz0344 (2020).
Park, D. et al. Crystal structure of proteolyzed VapBC and DNA-bound VapBC from Salmonella enterica Typhimurium LT2 and VapC as a putative Ca(2+) -dependent ribonuclease. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 3051-3068, doi:10.1096/fj.201901989R (2020).
연구부문
Application of nanoplatforms for biophysical studies of disease-associated membrane proteins and those in complex with drugs
Development of drug formulation and delivery systems using cutting-edge techniques
Development of new analytical platforms for the discovery of new drug candidates
